Sanitize the hands with 70 % v/v IPA. The precautions taken to avoid contamination must be such that they do not affect any microorganisms that are to be revealed in the test (water analysis). Annexure-2: Sampling Schedule of Purified Water. 1. Wear gloves and nose mask before sampling. 2. Chemical analysis: Microbiological Sampling and Testing May 2019 Science Staff Office of Public Health Science 1. Immediate resampling of the specific location where the OOT/OOS occurred must be performed, to rapidly assess the condition of the system. endobj These Alert levels can be modified depending on the trend analysis done in the monitoring program. Analyse the water samples for Microbiological analysis as per specifications. 3. If the immediate analysis is not possible to acidify the sample with a 2-3 drop of 2.0 M HCL and store the sample under refrigeration at 2 to 8ºC. %PDF-1.5 4 0 obj Ensure that the required contact time for the sanitizer has been achieved. Wrap the neck with aluminum foil to avoid any contamination during transportation. • In systems with more than one water source, the locations of the sampling points should take account of the number of inhabitants served by each source. This Standard Operating Procedure is applicable to the Microbiology Department. Carefully replace the cap. Water system sanitization methods require assessment. Head QA shall be responsible for the approval of SOP. 4.1.16 Again collect the swab sample and repeat procedure following Point 4.1.1 to 4.1.13. Avoid unpredictable changes in the microbial population ii. Sampling Instructions This method of sample collection is acceptable for EPA analytical methods 1622 and 1623 when sending water samples in to the laboratory without filtering in the field. 4.13 Carry out sampling step 4.14 and 4.19 as rapidly as possible to avoid exposure of the sample to the external environment. The final summary report shall be prepared at the end of the Year. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) Open tap and run to waste, allowing removal of any excess solution. A sample volume of 250 mL should be sufficient for Total coliform, Faecal coliform, E. coli and Heterotrophic plate count Please select the appropriate link in the table below to send your request via email, or contact Health Canada's publication office to make your request.. Note: Before sampling, the aluminum foil covering the sampling point should be removed. A sample volume of 200ml should be sufficient for Faecal coliform, E. coli and Heterotrophic plate count. Phase 1 focuses on the method and the analyst; the purpose of phase 1 is to verify that the data are valid. Microbial levels, specified in the standard operating procedure or specifications, when exceeded should result in an investigation to ensure that the process is still within control. Sampling apparatus and containers shall be clean, sterile, and/or endotoxin-free as per test requirements. Microbiologist shall wear sterilised gloves during the sampling activity. <> stream If sampling from a water faucet, run the faucet for 1 minute before obtaining a sample. x��[mo�6� �Aw�X_DIE�q�;�:M � Sampling Procedures for Drinking Waters 1 PURPOSE This standard operating procedure outlines details for collection of drinking water samples for chemical, microbiological and radiological analysis. for validated time. Moreover, there are many types of pathogen and each requires a unique microbiological isolation technique. Refer Annexure-2 for preparing the Sampling Schedule. Where potable (drinking water) water is stored, BS8558:2015 sets out a regime where 6 monthly microbiological activity is measured. Good water quality is important in many settings, including those found for all drinking water systems, during food production and in fresh- and seawater for recreation and bathing activity. There is a need to identify the source of contamination. Rinse with purified water. This document is not intended for public release. Close the valve and bring the samples to the laboratory and make an entry in the purified water inward register. Replace the used hose onto the provided clamp after sampling so that it fully drains. Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis … 7`rV�XH���ق�*�r��θ �NMsp�^%B�+$��3�V�&�y�J09;��������;�[KeR)����XA_�ώ�z5]�Un6)���\��ٻ���O���T�E���\J0^5�4?���xE��� Follow the procedures described below to assist in the collection of an acceptable sample and to maintain the integrity of the sample after collection. Analyze the sample of purified water for Total Viable Count using filtration technique as per the current specification of Purified Water. It represents one aspect of water quality. Carry the sampling containers to the sampling point as per the sampling schedule. – How can we meet the demands microbiological water AQC in the future? These procedures should be utilized by drinking water service providers. MICROBIOLOGICAL WATER SAMPLING - BASIC PROCEDURES Taking a Routine Bacteria Sample Properly Step One Do Not Rinse The Bottle The powder in the bottle is meant to be there and will not contaminate your sample. Microbiological Analysis of Water Quality •Looking in to the 21st Century sigma-aldrich.com January 31, 2012 By Richard Glindon Presented by Klaus Herick. Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. Close the container immediately and record the sampling time. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. Annexure-1: List of Sampling Points of Purified Water. Prepare a Sampling and Analysis Plan (SAP) which describes the sampling locations, numbers and types of samples to be If sampling a body of running water, point the mouth of the bag upstream and your hands downstream to avoid contamination. The Laboratory Procedures for the Microbiological Analysis of Foods are available upon request. Sampling water for subsequent analysis is often considered to be somewhat easier than sampling, say, soils for two main reasons: (i) water tends to be more homogeneous than soils, there is less point-to-point variability between two samples collected within the same vicinity, (ii) it is often physically easier to collect water samples because it can be done with pumps and hose lines, (iii) known volumes of water can … Open the valve of the sampling point and allow the water to drain for about 20-30 seconds. Water Sampling Procedure (with reference to ISO 5667 Part 5) (A) Assessment of Sampling Location Please take note of the conditions of the environment, sampling taps and pipes at the sampling location. Water column sampling using dedicated sampler devices specially designed to assess the impact of offshore activities on sea water quality Groundwater sampling Collection of groundwater and analysis to international standards using a comprehensive range of equipment, including interface meters, low and high flow sampling pumps, flow through cells and calibrated field instrumentation In particular, the spread of enteric viruses, e.g. Flush water through sampling point for 5 – 20 Seconds. Sampling … OBJECTIVE:To lay down a procedure for microbiological monitoring of raw water and purified water. These stored samples should be analyzed within 6 hours. If you would like the entire volume, please send your email request for a zip file.. 1 0 obj Wipe any visible dust from the exterior of the sample port or valve using a lint-free wipe/cloth moistened with an approved sanitizer – do not spray or wipe the interior of the port or valve. To lay down the procedure for Sampling and Testing of Purified Water. Where ever there is no drain carry the bucket to collect the water while flushing and avoid spillage of water on the floor. �d��5�ֹܵܯ�y!%r%��]iHg�3�g����U}�%�^u]}�l���Y{��������S}}����v��u���q�������""�trv���H2�O$F%�1�)���ϟe�5��ϟ�ϒ�o�ٿ�? ACoP L8 recommends that sampling for Legionella should be carried out when: for validated time. PROCEDURE: Sampling of water Sampling of water … Unwrap the aluminum foil and open the bottle taking care not to touch the inner surface and neck of the bottle. Vinlab supplies 100ml sterile plastic bottles. ISO 19458:2006 provides guidance on planning water sampling regimes, on sampling procedures for microbiological analysis and on transport, handling and storage of samples until analysis begins. RESPONSIBILITY Quality Control Executive / Officer ACCOUNTABILITY Quality Assurance Head. Head of the Department / Designee of respective areas & QA shall be responsible for reviewing the SOPs. Correct sampling procedures as instructed by international standard protocols must be adhered to; Routine microbiological testing of drinking water supplies, recreational waters, and environmental waters is essential for the protection of public health ; Programme of testing. It is important to note that you don’t have to exceed specification in order to submit a result for identification, it’s a best practice to document and select and identify representative flora from the system. After completion of the analytical report, checked and approved by a designated person and after that generate the COA (if required). Talk to the lab to determine if this collection procedure is acceptable for the analytical method they plan to perform. Phase 2 follows the determination of data validity and investigates the water system and all potentially affected lots of products. A designated person shall issue the template/worksheet. For Microbiological Analysis: Clean 250 ml Clear glass bottles with cap for membrane filtration method or pour plate method and rinse with purified water. ^���\�L �&�BϘ�B���X�d&ǣ=�K�}. 6.0 PROCEDURE FOR ANALYSIS OF WATER SAMPLE: Carry out the determination under conditions designed to avoid extrinsic microbial contamination of the water sample for the analysis. W|b1*]�y.����>'O�ak�Oߜ�~8� ���9X�;�����#|������O�en�4�O# Noroviruses, are repeatedly related to poor water quality (… Utility system sampling requires additional care because samples are collected in controlled and uncontrolled areas of the facility. Collect the purified water sample (refer table 1) without rinsing and close it with stopper immediately. Faecal contamination of water is routinely detected by microbiological analysis. Alert levels are always lower than Action levels. Flush the sample port/valve for the qualified time or volume and immediately open the labeled sample container and collect the sample. Step Two Wash Your Hands prior to taking the sample. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. Fill the bottle almost to the top, leaving a gap for mixing (approx 5 mm). The sampling procedures and … Water quality — Sampling for microbiological analysis. If the hose is not connected but is provided, attach the hose using procedures applicable for that system. Preparation of Containers for the sampling of purified water: Clean 1000 ml Clear glass bottles with a cap. Rinse the container at least one to two times with purified water from the sampling point. 4.12 Control the valve of sampling point to avoid splashing. PROCEDURE FOR MICROBIOLOGICAL MONITORING OF WATER. 3 0 obj {cy�L���*3i��S��e�T�����4�VS�H�¤y��2��d�����4(�"^�xnfg���kS��p�'�T�@�ů�_�:�}��$w��>�&���=������o3!�J� �H�bZ� Carry out the chemical analysis of samples as per the current version of purified water specification. After completion of the test, record the results in the datasheet and also enter the results in the respective software/module. SCOPE:This SOP shall provide the procedure for sampling and testing of raw water and purified water from all the user points and all the points across the critical functions in the water purification process. • Sampling points should be located in such a way that water can be sampled from reserve tanks and reservoirs, etc. Collect your water sample early in the week – Monday to Thursday –to give enough time to get to the laboratory and to be analyzed within 24-30 hours. Approximately add 15-20 ml of R2A / Plate count Agar into each Petri dishes. Officer or Executive of the Microbiology department shall be responsible for the preparation of new or revision of existing SOP. If a hose is already connected to the port or valve, collect the sample directly from that hose. Ensure you have read the ‘Standard drinking water sampling procedure – microbiological’. Wash 125 ml Amber glass or suitable bottles with a stopper with 2.0 M HCL or 2.0% nitric acid and rinse with purified water and dry the bottles at 105 ºC for at least one hour. Domestic water systems are supplied with water fit to drink, so ACoP L8 advices that regular microbiological sampling is not required. 2 0 obj If the immediate analysis is not possible, store the sample under refrigeration at 2 to 8ºC. The changes may occur in the bacterial content of water on storage can be reduced to a minimum by ensuring that samples are not exposed to light and are kept cool. Step Three Remove Faucet Screen and Rinse the bag twice with the sample water prior to filling and closing. These guidelines collate and standardise PathWest’s microbiological analysis methods as part of the sampling, testing and analysis agreement between Western Australian Local Governments and the Department of Health. 4.2 Rinse water sampling: 4.2.1 After cleaning, the Production Officer shall inform to QA Officer for the collection of the rinse water sample. drinking water samples and handling of sample preservatives. Write the sampling details or affix the label on the bottle as per Annexure-3. make the schedule in such manner that generation point (For Chemical & Microbiology Analysis) & return loop (For Microbiology Analysis only) covered on a daily basis and other useful points (For Microbiology Analysis only) to be covered in rotation within a month. Food Safety and Inspection Service: •Sampling methods •Assessing sampling plans and testing methods •Method validations and laboratory quality assurance •FSIS testing programs, methods and pathogen-specific issues to consider •Industry testing activities 2 Today’s Presentation. Perform the analysis as per the current version of purified water specification. 2. Wear gloves when collecting samples. If no sterile bottle is available, rinse the bottle first with hot water, then with the sample and then proceed to fill the bottle with sample. PathWest Laboratory Medicine WA Manual: Water Examination Procedures Title: Collection And Transportation Instructions For Water Sampling Document Number: MWP119 Version Number: 1.7 Document Owner: Woodward, Robin Page 1 of 7 Date Issued: 14-Dec-2017 COLLECTION AND TRANSPORTATION INSTRUCTIONS FOR MICROBIOLOGICAL WATER SAMPLING 1. It is impractical to attempt the routine isolation of pathogens because they are present in relatively small numbers compared with other types of micro-organism. Prepare the sampling schedule (Tentative) by the first working day of every New Year. 2 Content • Introduction – What does the future hold for microbiological water AQC? Alert levels are specific for a given facility and are established on the basis of a baseline developed under an activity monitoring program. Close the bottle with a cap and wrap the neck with aluminum foil and Sterilize the bottle at 121 ºC (15 lbs.) If any chemical parameter exceeds the limit, immediately inform the same QA, Production, and Engineering Department. 1 minute. endobj Fill the containers with 1000 ml and 125 ml or up to the neck of purified water for chemical and TOC analysis respectively, and close the lid immediately. • There should be at least one sampling point directly after the clean-water If the following situation(s) is/are encountered, DO NOT TAKE SAMPLES. Use sodium hypochlorite solution (1g/L free chlorine) or ethanol/isopropanol 70% v/v. surroundings dusty, covered with debris, or poorly ventilated; 2. HOW TO COLLECT A WATER SAMPLE FOR MICROBIOLOGY EXAMINATION Created: March 26, 2015 Page 1 of 2 *Conditions Apply 1. Aseptic Technique for Microbiological Testing. Purified water quality parameter trending shall be done on the Monthly basis, trend charts shall be reviewed and a conclusion shall be made (if required). Analyze the sample of purified water for pathogens using filtration technique/Direct as per the current specification of Purified water. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Analyze the sample as quickly as possible on arrival at the laboratory. SAMPLING FOR MICROBIOLOGICAL ANALYSIS In case of laboratory analysis for microbiological purpose, following guidelines are adopted to collect representative water samples: Sample Bottles Pre-sterilized sampling bottles of 200 ml capacity made of pre-sterilized disposible/autoclavable plastic, or good quality soda / borosilicate glass. ) is/are encountered, DO not TAKE samples of respective areas & QA shall be responsible for preparation... Because samples are collected in Pre-sterilized screw cap glass bottles which Vinlab supplies sterile, and/or endotoxin-free per! 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